TLS provides security to LDAP communications by implementing SSL. By default, TLS is enabled on a new LDAP connection. When unchecking the ‘Use TLS’ option, you may see the warning “Warning - LDAP should not be used without TLS other than for diagnostic purposes. This is highly insecure.”
What is TLS:
Transport Layer Security (TLS) is a protocol that provides privacy and data integrity between two communicating applications. It's the most widely deployed security protocol used today, and is used for Web browsers and other applications that require data to be securely exchanged over a network, such as file transfers, VPN connections, instant messaging and voice over IP
The TLS Handshake Protocol allows the server and client to authenticate each other and to negotiate an encryption algorithm and cryptographic keys before data is exchanged. In a typical scenario, only the server is authenticated and its identity is ensured while the client remains unauthenticated. The mutual authentication of the servers requires public key deployment to clients. When a server and client communicate, TLS protocol ensures that no third party may eavesdrop, tamper with any message, and message forgery

For information on preparing a Windows server for LDAP communication with TLS, see:
How to Integrate LDAP over TLS with Windows Server 2012
Integrating LDAP over TLS in SonicOS Enhanced with Windows Server 2008